Post date: 3/11/19
We use the mouse as a paradigm for investigating the developmental biology of mammalian gametogenesis and
early embryogenesis. Our studies focus on molecular mechanisms of sex-specific factors that: 1) promote formation
of ovulated eggs and mature spermatozoa; 2) ensure fertilization; and 3) support early development.
Gametogenesis: A hallmark of female germ cell development is asymmetric cell divisions during meiosis that
result in large, ovulated eggs containing maternal factors necessary for early development. A hallmark of male
germ cells is post-meiotic spermiogenesis in which round spermatids are transmogrified into mature, motile
spermatozoa capable of fertilization. We have identified entrance points into genetic hierarchies that we exploit
to investigate the networks that control these pathways.
Fertilization: Monospermic fertilization is essential for the onset of mammalian development. One sperm is required,
but two are embryonic lethal. A major arbiter of this constricted window of opportunity is the zona pellucida that
surrounds ovulated eggs and pre-implantation embryos. Molecular biology and gene-editing in mice are used to validate
models of gamete recognition, learn more about cortical granule biology and investigate the maintenance of monospermy.
Early Development: We study the egg to embryo transition by investigating maternal effect genes that encode proteins
required for pre-implantation development. We also investigate degradation of maternal proteins and RNA on which
successful embryogenesis is dependent.
The lab occupies modern, well-equipped space on the Bethesda campus of the NIH replete with a procedure room in the
vivarium with integrated systems for oocyte, pronuclear and blastocyst injections. The technologies necessary to carry
out our research are well established and the presence of a highly experienced embryologist, an imaging technologist,
and embedded bioinformatic support ensure success in making and analyzing the phenotype of transgenic and gene-edited
mice. Mentoring is emphasized and fellows are expected to develop independence by pursing their projects to fruition
during their training at the NIH.
About You
Ideal candidates will be trained in current DNA/RNA methodologies and have less than 5 years of postdoctoral experience.
Contact Dr. Jurrien DeanBack to All Jobs and Recruitment
